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June 24 2015

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One Morning at a Dig Site: A Cartoon by Jason Yungbluth
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  Hotline Miami (2) is coming
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June 19 2015

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Winter is coming. But first, let me take a selfie.
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“To see a World in a Grain of Sand
And a Heaven in a Wild Flower,
Hold Infinity in the palm of your hand
And Eternity in an hour.”  
~ William Blake ~

Animation by : google.com/+DarkAngel0ne

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May 31 2015

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Nice try
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May 30 2015

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I wish this was longer

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May 24 2015

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A painting by Peter Elson (based on a design by Mike Trim) for the liner notes of Jeff Wayne’s War of the Worlds album.

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May 22 2015

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Ronald Campbell Macfie: Metanthropos, or, The body of the future. (1928)

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
by Puping Liang, Yanwen Xu, Xiya Zhang et al. (Guangzhou, China)
Protein & Cell, May 2015, Volume 6, Issue 5, pp 363-372,

Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISPR/Cas9 in human pre-implantation embryos. In this report, we used tripronuclear (3PN) zygotes to further investigate CRISPR/Cas9-mediated gene editing in human cells. We found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB). However, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic. Off-target cleavage was also apparent in these 3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing. Furthermore, the endogenous delta-globin gene (HBD), which is homologous to HBB, competed with exogenous donor oligos to act as the repair template, leading to untoward mutations. Our data also indicated that repair of the HBB locus in these embryos occurred preferentially through the non-crossover HDR pathway. Taken together, our work highlights the pressing need to further improve the fidelity and specificity of the CRISPR/Cas9 platform, a prerequisite for any clinical applications of CRSIPR/Cas9-mediated editing.

Full text: http://link.springer.com/content/pdf/10.1007%2Fs13238-015-0153-5.pdf
Reposted fromArchimedes Archimedes viascience science
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